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sox17  (Miltenyi Biotec)


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    Miltenyi Biotec sox17
    Sox17, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sox17/Sox17+Antibody%2C+anti-human%2C+REAfinity/pm42034633-353-11-28
    Average 94 stars, based on 11 article reviews
    sox17 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Sequencing:

    Article Title: Generation of a homozygous and heterozygous iPSC line carrying a variant of uncertain significance in CACNA1C, associated with Brugada syndrome.
    Article Snippet: .. Sanger sequencing of top predicted off-target sites, identical to reference genome Supplementary Fig. 2 Specific pathogen-free status Mycoplasma Negative (PCR) Supplementary Fig. 3 Characterization of the differentiation potential Directed tri-lineage differentiation StemMACS Trilineage Differentiation Kit (Miltenyi Biotec) Fig. 1 panel D List of recommended germ layer markers Expression of germ layer markers (RT-PCR) RT-qPCR confirmation: endoderm markers : SOX17, FOXA2 & CXCR4; mesoderm markers: HAND1 & NKX2.5; Ectoderm markers: PAX6, HES5, MAP2 Fig. 1 panel D Overall outcomes of gene editing experiment (OPTIONAL) Brief description of the outcomes in terms of clones generated / establishment approach / editing event screening outcomes N/A N/A Donor screening (OPTIONAL) HIV 1 + 2 Hepatitis B, Hepatitis C N/A N/A Genotype − additional histocompatibility info (OPTIONAL) Blood group genotyping N/A N/A HLA tissue typing N/A N/A B. Vandendriessche et al. ..

    Polymerase Chain Reaction:

    Article Title: Generation of a homozygous and heterozygous iPSC line carrying a variant of uncertain significance in CACNA1C, associated with Brugada syndrome.
    Article Snippet: .. Sanger sequencing of top predicted off-target sites, identical to reference genome Supplementary Fig. 2 Specific pathogen-free status Mycoplasma Negative (PCR) Supplementary Fig. 3 Characterization of the differentiation potential Directed tri-lineage differentiation StemMACS Trilineage Differentiation Kit (Miltenyi Biotec) Fig. 1 panel D List of recommended germ layer markers Expression of germ layer markers (RT-PCR) RT-qPCR confirmation: endoderm markers : SOX17, FOXA2 & CXCR4; mesoderm markers: HAND1 & NKX2.5; Ectoderm markers: PAX6, HES5, MAP2 Fig. 1 panel D Overall outcomes of gene editing experiment (OPTIONAL) Brief description of the outcomes in terms of clones generated / establishment approach / editing event screening outcomes N/A N/A Donor screening (OPTIONAL) HIV 1 + 2 Hepatitis B, Hepatitis C N/A N/A Genotype − additional histocompatibility info (OPTIONAL) Blood group genotyping N/A N/A HLA tissue typing N/A N/A B. Vandendriessche et al. ..

    Expressing:

    Article Title: Generation of a homozygous and heterozygous iPSC line carrying a variant of uncertain significance in CACNA1C, associated with Brugada syndrome.
    Article Snippet: .. Sanger sequencing of top predicted off-target sites, identical to reference genome Supplementary Fig. 2 Specific pathogen-free status Mycoplasma Negative (PCR) Supplementary Fig. 3 Characterization of the differentiation potential Directed tri-lineage differentiation StemMACS Trilineage Differentiation Kit (Miltenyi Biotec) Fig. 1 panel D List of recommended germ layer markers Expression of germ layer markers (RT-PCR) RT-qPCR confirmation: endoderm markers : SOX17, FOXA2 & CXCR4; mesoderm markers: HAND1 & NKX2.5; Ectoderm markers: PAX6, HES5, MAP2 Fig. 1 panel D Overall outcomes of gene editing experiment (OPTIONAL) Brief description of the outcomes in terms of clones generated / establishment approach / editing event screening outcomes N/A N/A Donor screening (OPTIONAL) HIV 1 + 2 Hepatitis B, Hepatitis C N/A N/A Genotype − additional histocompatibility info (OPTIONAL) Blood group genotyping N/A N/A HLA tissue typing N/A N/A B. Vandendriessche et al. ..

    Quantitative RT-PCR:

    Article Title: Generation of a homozygous and heterozygous iPSC line carrying a variant of uncertain significance in CACNA1C, associated with Brugada syndrome.
    Article Snippet: .. Sanger sequencing of top predicted off-target sites, identical to reference genome Supplementary Fig. 2 Specific pathogen-free status Mycoplasma Negative (PCR) Supplementary Fig. 3 Characterization of the differentiation potential Directed tri-lineage differentiation StemMACS Trilineage Differentiation Kit (Miltenyi Biotec) Fig. 1 panel D List of recommended germ layer markers Expression of germ layer markers (RT-PCR) RT-qPCR confirmation: endoderm markers : SOX17, FOXA2 & CXCR4; mesoderm markers: HAND1 & NKX2.5; Ectoderm markers: PAX6, HES5, MAP2 Fig. 1 panel D Overall outcomes of gene editing experiment (OPTIONAL) Brief description of the outcomes in terms of clones generated / establishment approach / editing event screening outcomes N/A N/A Donor screening (OPTIONAL) HIV 1 + 2 Hepatitis B, Hepatitis C N/A N/A Genotype − additional histocompatibility info (OPTIONAL) Blood group genotyping N/A N/A HLA tissue typing N/A N/A B. Vandendriessche et al. ..

    Clone Assay:

    Article Title: Generation of a homozygous and heterozygous iPSC line carrying a variant of uncertain significance in CACNA1C, associated with Brugada syndrome.
    Article Snippet: .. Sanger sequencing of top predicted off-target sites, identical to reference genome Supplementary Fig. 2 Specific pathogen-free status Mycoplasma Negative (PCR) Supplementary Fig. 3 Characterization of the differentiation potential Directed tri-lineage differentiation StemMACS Trilineage Differentiation Kit (Miltenyi Biotec) Fig. 1 panel D List of recommended germ layer markers Expression of germ layer markers (RT-PCR) RT-qPCR confirmation: endoderm markers : SOX17, FOXA2 & CXCR4; mesoderm markers: HAND1 & NKX2.5; Ectoderm markers: PAX6, HES5, MAP2 Fig. 1 panel D Overall outcomes of gene editing experiment (OPTIONAL) Brief description of the outcomes in terms of clones generated / establishment approach / editing event screening outcomes N/A N/A Donor screening (OPTIONAL) HIV 1 + 2 Hepatitis B, Hepatitis C N/A N/A Genotype − additional histocompatibility info (OPTIONAL) Blood group genotyping N/A N/A HLA tissue typing N/A N/A B. Vandendriessche et al. ..

    Generated:

    Article Title: Generation of a homozygous and heterozygous iPSC line carrying a variant of uncertain significance in CACNA1C, associated with Brugada syndrome.
    Article Snippet: .. Sanger sequencing of top predicted off-target sites, identical to reference genome Supplementary Fig. 2 Specific pathogen-free status Mycoplasma Negative (PCR) Supplementary Fig. 3 Characterization of the differentiation potential Directed tri-lineage differentiation StemMACS Trilineage Differentiation Kit (Miltenyi Biotec) Fig. 1 panel D List of recommended germ layer markers Expression of germ layer markers (RT-PCR) RT-qPCR confirmation: endoderm markers : SOX17, FOXA2 & CXCR4; mesoderm markers: HAND1 & NKX2.5; Ectoderm markers: PAX6, HES5, MAP2 Fig. 1 panel D Overall outcomes of gene editing experiment (OPTIONAL) Brief description of the outcomes in terms of clones generated / establishment approach / editing event screening outcomes N/A N/A Donor screening (OPTIONAL) HIV 1 + 2 Hepatitis B, Hepatitis C N/A N/A Genotype − additional histocompatibility info (OPTIONAL) Blood group genotyping N/A N/A HLA tissue typing N/A N/A B. Vandendriessche et al. ..

    FACS:

    Article Title: SteMClass: A Novel DNA Methylation-Based Classifier for iPSC In Vitro Differentiation States
    Article Snippet: .. At day 3 cells were harvested using Accutase and immunostained for FACS analysis using CD184 (CXCR4) (Miltenyi Biotec, 130-120-708) and SOX17 (Miltenyi Biotec, 130-111-031). ..

    Article Title: Recapitulating thyroid cancer histotypes through engineering embryonic stem cells.
    Article Snippet: DNA content was evaluated by BD FACS Lyric flow cytometer (BD Clinical system, BD Biosciences). .. For FACS analysis, hESCs-derived TPCs were washed in PBS and exposed for 1 h at 4 °C to CD44v6 (2F10 APC, mouse IgG1, R&D systems, 5μl/sample), Oct3/4 (40/Oct-3 Alexa-fluor647, mouse IgG1K, BD Biosciences, 20μl/sample), Sox2 (245610 PE, mouse IgG2a BD Biosciences, 20μl/sample), Nanog (N31-355 PE, mouse IgG1, BD Biosciences, 20μl/sample), CXCR4 (FAB170P PE, mouse IgG2a, R&D system, 1μl/sample), c-kit (YB5.B8 PE, mouse IgG1 BD Biosciences, 20μl/sample), Sox17 (P7-969 PE, mouse IgG1k, BD Biosciences, 5μl/ sample), FoxA2 (N17-280 PE,mouse IgG1, BDBiosciences 5μl/sample), PAX8 (PAX8/1492 APC, mouse IGg2a, Novus 5μl/sample), TTF1 (REA1090 FITC, mouse IgG1, MACS Miltenyi Biotec, 16μl/sample), TSH-R (4C1 FITC, IgG2a Santa Cruz, 2μl/sample), TPO (203340, Antirabbit IgG H + L Alexa-488, Abcam, 2μl/sample), Thyroglobulin (SPM221 PE, mouse IgG1, Novus, 2μl/sample), NIS (SPM186, goat antimouse IgG (H + L) Alexa-488, Abcam, 5μl/sample), CD133 (W6B3C1 FITC, mouse IgG1, BD Bioscience, 20μl/sample), ABCG2 (5D3/CD338 APC, mouse IgG2b, BD Bioscience, 10μl/sample), Nestin (25/Nestin PerCP 5.5, mouse IgG1, BD Bioscience, 5μl/sample), HNF-4α (H-1, goat anti-mouse IgG (H+ L) Alexa-488, Santa Cruz, 4μl/sample), or corresponding isotypematched controls (IMC) and analyzedusing the FACS Lyric cytometer (BD Biosciences). ..

    Article Title: Recapitulating thyroid cancer histotypes through engineering embryonic stem cells
    Article Snippet: DNA content was evaluated by BD FACS Lyric flow cytometer (BD Clinical system, BD Biosciences). .. For FACS analysis, hESCs-derived TPCs were washed in PBS and exposed for 1 h at 4 °C to CD44v6 (2F10 APC, mouse IgG1, R&D systems, 5 μl/sample), Oct3/4 (40/Oct-3 Alexa-fluor647, mouse IgG1K, BD Biosciences, 20 μl/sample), Sox2 (245610 PE, mouse IgG2a BD Biosciences, 20 μl/sample), Nanog (N31-355 PE, mouse IgG1, BD Biosciences, 20 μl/sample), CXCR4 (FAB170P PE, mouse IgG2a, R&D system, 1 μl/sample), c-kit (YB5.B8 PE, mouse IgG1 BD Biosciences, 20 μl/sample), Sox17 (P7-969 PE, mouse IgG1k, BD Biosciences, 5 μl/sample), Fox A2 (N17-280 PE, mouse IgG1, BD Biosciences 5 μl/sample), PAX8 (PAX8/1492 APC, mouse IGg2a, Novus 5 μl/sample), TTF1 (REA1090 FITC, mouse IgG1, MACS Miltenyi Biotec, 16 μl/sample), TSH-R (4C1 FITC, IgG2a Santa Cruz, 2 μl/sample), TPO (203340, Antirabbit IgG H + L Alexa-488, Abcam, 2 μl/sample), Thyroglobulin (SPM221 PE, mouse IgG1, Novus, 2 μl/sample), NIS (SPM186, goat antimouse IgG (H + L) Alexa-488, Abcam, 5 μl/sample), CD133 (W6B3C1 FITC, mouse IgG1, BD Bioscience, 20 μl/sample), ABCG2 (5D3/CD338 APC, mouse IgG2b, BD Bioscience, 10 μl/sample), Nestin (25/Nestin PerCP 5.5, mouse IgG1, BD Bioscience, 5 μl/sample), HNF-4α (H-1, goat anti-mouse IgG (H + L) Alexa-488, Santa Cruz, 4 μl/sample), or corresponding isotype matched controls (IMC) and analyzed using the FACS Lyric cytometer (BD Biosciences). ..

    Magnetic Cell Separation:

    Article Title: Recapitulating thyroid cancer histotypes through engineering embryonic stem cells.
    Article Snippet: DNA content was evaluated by BD FACS Lyric flow cytometer (BD Clinical system, BD Biosciences). .. For FACS analysis, hESCs-derived TPCs were washed in PBS and exposed for 1 h at 4 °C to CD44v6 (2F10 APC, mouse IgG1, R&D systems, 5μl/sample), Oct3/4 (40/Oct-3 Alexa-fluor647, mouse IgG1K, BD Biosciences, 20μl/sample), Sox2 (245610 PE, mouse IgG2a BD Biosciences, 20μl/sample), Nanog (N31-355 PE, mouse IgG1, BD Biosciences, 20μl/sample), CXCR4 (FAB170P PE, mouse IgG2a, R&D system, 1μl/sample), c-kit (YB5.B8 PE, mouse IgG1 BD Biosciences, 20μl/sample), Sox17 (P7-969 PE, mouse IgG1k, BD Biosciences, 5μl/ sample), FoxA2 (N17-280 PE,mouse IgG1, BDBiosciences 5μl/sample), PAX8 (PAX8/1492 APC, mouse IGg2a, Novus 5μl/sample), TTF1 (REA1090 FITC, mouse IgG1, MACS Miltenyi Biotec, 16μl/sample), TSH-R (4C1 FITC, IgG2a Santa Cruz, 2μl/sample), TPO (203340, Antirabbit IgG H + L Alexa-488, Abcam, 2μl/sample), Thyroglobulin (SPM221 PE, mouse IgG1, Novus, 2μl/sample), NIS (SPM186, goat antimouse IgG (H + L) Alexa-488, Abcam, 5μl/sample), CD133 (W6B3C1 FITC, mouse IgG1, BD Bioscience, 20μl/sample), ABCG2 (5D3/CD338 APC, mouse IgG2b, BD Bioscience, 10μl/sample), Nestin (25/Nestin PerCP 5.5, mouse IgG1, BD Bioscience, 5μl/sample), HNF-4α (H-1, goat anti-mouse IgG (H+ L) Alexa-488, Santa Cruz, 4μl/sample), or corresponding isotypematched controls (IMC) and analyzedusing the FACS Lyric cytometer (BD Biosciences). ..

    Article Title: Recapitulating thyroid cancer histotypes through engineering embryonic stem cells
    Article Snippet: DNA content was evaluated by BD FACS Lyric flow cytometer (BD Clinical system, BD Biosciences). .. For FACS analysis, hESCs-derived TPCs were washed in PBS and exposed for 1 h at 4 °C to CD44v6 (2F10 APC, mouse IgG1, R&D systems, 5 μl/sample), Oct3/4 (40/Oct-3 Alexa-fluor647, mouse IgG1K, BD Biosciences, 20 μl/sample), Sox2 (245610 PE, mouse IgG2a BD Biosciences, 20 μl/sample), Nanog (N31-355 PE, mouse IgG1, BD Biosciences, 20 μl/sample), CXCR4 (FAB170P PE, mouse IgG2a, R&D system, 1 μl/sample), c-kit (YB5.B8 PE, mouse IgG1 BD Biosciences, 20 μl/sample), Sox17 (P7-969 PE, mouse IgG1k, BD Biosciences, 5 μl/sample), Fox A2 (N17-280 PE, mouse IgG1, BD Biosciences 5 μl/sample), PAX8 (PAX8/1492 APC, mouse IGg2a, Novus 5 μl/sample), TTF1 (REA1090 FITC, mouse IgG1, MACS Miltenyi Biotec, 16 μl/sample), TSH-R (4C1 FITC, IgG2a Santa Cruz, 2 μl/sample), TPO (203340, Antirabbit IgG H + L Alexa-488, Abcam, 2 μl/sample), Thyroglobulin (SPM221 PE, mouse IgG1, Novus, 2 μl/sample), NIS (SPM186, goat antimouse IgG (H + L) Alexa-488, Abcam, 5 μl/sample), CD133 (W6B3C1 FITC, mouse IgG1, BD Bioscience, 20 μl/sample), ABCG2 (5D3/CD338 APC, mouse IgG2b, BD Bioscience, 10 μl/sample), Nestin (25/Nestin PerCP 5.5, mouse IgG1, BD Bioscience, 5 μl/sample), HNF-4α (H-1, goat anti-mouse IgG (H + L) Alexa-488, Santa Cruz, 4 μl/sample), or corresponding isotype matched controls (IMC) and analyzed using the FACS Lyric cytometer (BD Biosciences). ..

    Article Title: Protocol for generation and engineering of thyroid cell lineages using CRISPR-Cas9 editing to recapitulate thyroid cancer histotype progression.
    Article Snippet: Pantina et al., STAR Protocols 5, 103263 September 20, 2024 a 2024 The Author(s).. Published by Thyroid carcinoma represents the first malignancy among the endocrine organs.. Investigating the cellular hierarchy and the mechanisms underlying the initiation of thyroid carcinoma is crucial in thyroid cancer research.

    Cytometry:

    Article Title: Recapitulating thyroid cancer histotypes through engineering embryonic stem cells.
    Article Snippet: DNA content was evaluated by BD FACS Lyric flow cytometer (BD Clinical system, BD Biosciences). .. For FACS analysis, hESCs-derived TPCs were washed in PBS and exposed for 1 h at 4 °C to CD44v6 (2F10 APC, mouse IgG1, R&D systems, 5μl/sample), Oct3/4 (40/Oct-3 Alexa-fluor647, mouse IgG1K, BD Biosciences, 20μl/sample), Sox2 (245610 PE, mouse IgG2a BD Biosciences, 20μl/sample), Nanog (N31-355 PE, mouse IgG1, BD Biosciences, 20μl/sample), CXCR4 (FAB170P PE, mouse IgG2a, R&D system, 1μl/sample), c-kit (YB5.B8 PE, mouse IgG1 BD Biosciences, 20μl/sample), Sox17 (P7-969 PE, mouse IgG1k, BD Biosciences, 5μl/ sample), FoxA2 (N17-280 PE,mouse IgG1, BDBiosciences 5μl/sample), PAX8 (PAX8/1492 APC, mouse IGg2a, Novus 5μl/sample), TTF1 (REA1090 FITC, mouse IgG1, MACS Miltenyi Biotec, 16μl/sample), TSH-R (4C1 FITC, IgG2a Santa Cruz, 2μl/sample), TPO (203340, Antirabbit IgG H + L Alexa-488, Abcam, 2μl/sample), Thyroglobulin (SPM221 PE, mouse IgG1, Novus, 2μl/sample), NIS (SPM186, goat antimouse IgG (H + L) Alexa-488, Abcam, 5μl/sample), CD133 (W6B3C1 FITC, mouse IgG1, BD Bioscience, 20μl/sample), ABCG2 (5D3/CD338 APC, mouse IgG2b, BD Bioscience, 10μl/sample), Nestin (25/Nestin PerCP 5.5, mouse IgG1, BD Bioscience, 5μl/sample), HNF-4α (H-1, goat anti-mouse IgG (H+ L) Alexa-488, Santa Cruz, 4μl/sample), or corresponding isotypematched controls (IMC) and analyzedusing the FACS Lyric cytometer (BD Biosciences). ..

    Article Title: Recapitulating thyroid cancer histotypes through engineering embryonic stem cells
    Article Snippet: DNA content was evaluated by BD FACS Lyric flow cytometer (BD Clinical system, BD Biosciences). .. For FACS analysis, hESCs-derived TPCs were washed in PBS and exposed for 1 h at 4 °C to CD44v6 (2F10 APC, mouse IgG1, R&D systems, 5 μl/sample), Oct3/4 (40/Oct-3 Alexa-fluor647, mouse IgG1K, BD Biosciences, 20 μl/sample), Sox2 (245610 PE, mouse IgG2a BD Biosciences, 20 μl/sample), Nanog (N31-355 PE, mouse IgG1, BD Biosciences, 20 μl/sample), CXCR4 (FAB170P PE, mouse IgG2a, R&D system, 1 μl/sample), c-kit (YB5.B8 PE, mouse IgG1 BD Biosciences, 20 μl/sample), Sox17 (P7-969 PE, mouse IgG1k, BD Biosciences, 5 μl/sample), Fox A2 (N17-280 PE, mouse IgG1, BD Biosciences 5 μl/sample), PAX8 (PAX8/1492 APC, mouse IGg2a, Novus 5 μl/sample), TTF1 (REA1090 FITC, mouse IgG1, MACS Miltenyi Biotec, 16 μl/sample), TSH-R (4C1 FITC, IgG2a Santa Cruz, 2 μl/sample), TPO (203340, Antirabbit IgG H + L Alexa-488, Abcam, 2 μl/sample), Thyroglobulin (SPM221 PE, mouse IgG1, Novus, 2 μl/sample), NIS (SPM186, goat antimouse IgG (H + L) Alexa-488, Abcam, 5 μl/sample), CD133 (W6B3C1 FITC, mouse IgG1, BD Bioscience, 20 μl/sample), ABCG2 (5D3/CD338 APC, mouse IgG2b, BD Bioscience, 10 μl/sample), Nestin (25/Nestin PerCP 5.5, mouse IgG1, BD Bioscience, 5 μl/sample), HNF-4α (H-1, goat anti-mouse IgG (H + L) Alexa-488, Santa Cruz, 4 μl/sample), or corresponding isotype matched controls (IMC) and analyzed using the FACS Lyric cytometer (BD Biosciences). ..

    other:

    Article Title: Non-destructive transcriptomics via vesicular export
    Article Snippet: The following antibodies were used: PAX6 (APC, REAfinityTM), SOX2 (FITC, REAfinityTM), SOX17 (APC, REAfinityTM), CD184/CXCR4 (Vio® Bright FITC, REAfinityTM), CD144/VE-cadherin (FITC, REAfinityTM), and CD140b (APC, REAfinityTM) (all anti-human; Miltenyi Biotec).

    Staining:

    Article Title: Generation of three myotonic dystrophy type 1 patient iPSC lines (CBRCULi018-A, CBRCULi019-A, CBRCULi020-A) derived from lymphoblastoid cell lines for disease modelling and therapeutic research.
    Article Snippet: Viability assessment was conducted by staining cells (10x106/mL) with BD HorizonTM Fixable Viability Stain 780 (BD Biosciences) and antibody staining was carried out at 5x105 cells/mL. .. Staining for SOX2, PAX6, SOX17, and CXCR4 utilized the Foxp3 Staining Buffer Set (Miltenyi Biotec). .. DNA was extracted from passage 4–6 iPSCs using Quick-RNA kits (Zymo Research).



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